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Chromium-catechin complex was synthesized by reacting [Cr(H2O)6]2+ (hexa-aqua) with catechin as a ligand. Toxicity studies were carried out for the complex using bacterial models for safer application of this complex in the future as a drug. https://www.selleckchem.com/products/MK-1775.html Chromium-catechin complex was characterized using ESI Mass spectrometry, electronic spectroscopy, FT-IR spectroscopy and cyclic voltammetry. The complex was found mildly inhibitory towards B. subtilis with the mode of action being oxidative damage, targeting cell membrane. The complex was supportive towards E. coli, which was evident from the growth profile and inhibition studies. SEM analysis supported the results of membrane integrity studies, where the bacterial liposomes upon treatment with the complex revealed slight morphological changes in the case of B. subtilis, without any change in the case of E. coli. The toxicity studies on chromium-catechin complex using bacterial model saves time, as well as resources by providing quick and reliable results, which could ease up the work to be done in future with higher group of organisms like animal model. Therefore, in the future, this complex can be used as an antidiabetic drug after performing toxicity studies with animal model.In the present study, silver nanoparticles (AgNPs) were synthesized using aqueous leaf extracts of three Congolese plant species, namely Brillantaisia patula (BR-PA), Crossopteryx febrifuga (CR-FE) and Senna siamea (SE-SI). The obtained AgNPs were studied for their optical, structural, surface morphological and antibacterial properties. The prepared AgNPs were characterized by using UV-Visible spectra, Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray spectroscopy (EDX) and X-ray diffractometer (XRD). The synthesized nanoparticles were spherical shaped and well-dispersed with average sizes ranging from 45 to 110 nm. The AgNPs derived from BR-PA, CR-FE and SE-SI exhibited higher antibacterial activity against three bacterial pathogens of the human skin compared to their respective crude extracts and AgNO3. This indicated that the biomolecules covering the nanoparticles may enhance the biological activity of metal nanoparticles. Hence, our results support that biogenic synthesis of AgNPs from Congolese plants constitutes a potential area of interest for the therapeutic management of microbial diseases such as infectious skin diseases.In response to the need for quantitative instruments that can provide insights into language teacher multilingualism on a large scale, this article discusses the development of the MULTITEACH questionnaire via a five-stage process that consisted of a critical review of research on teacher multilingualism, seminar and practitioner consultations, a pilot study, reliability tests, and principle component analysis (PCA), followed by a larger study involving 460 multilingual language teachers and factor analysis to confirm the PCA. The questionnaire thus validated provides a comprehensive quantitative measure of assessing language teacher multilingualism across multiple foreign languages and in diverse contexts. A discussion of the factors influencing teacher multilingualism has also been included based on the findings from the larger study of 460 multilingual teachers.In present work, ordered mesoporous material SBA-15 was synthesized by using poly (alkylene oxide) block copolymer (Pluronic P123) as template and ethylsilicate as silica source in weak acid environment in a wide range of temperature. The focus of synthesis research was high hydrothermal temperature. The obtained products were characterized by various techniques, including XRD, N2 sorption isotherms, FTIR spectroscopy and thermogravimetric. The effect of hydrothermal temperature on the specific surface area, pore volume and pore size of SBA-15 products was investigated systematically. As the hydrothermal temperature increases from the 100-120 °C, the specific surface area and the pore volume of the mesoporous molecular sieve increase greatly. When the hydrothermal temperature increase further, the pore volume of the mesoporous molecular sieve increase continually. But the specific surface area decrease significantly. When the hydrothermal temperature is too high (over 140 °C), the order degree begins to decrease, So the specific surface area and pore volume decrease significantly because the pores structure have significant destruction and collapse. Mechanism and structural characteristics of P123 block copolymer could explain in detail the effect of hydrothermal temperature on the property and structure of mesoporous molecular sieve SBA-15.One of the main factors in the success of projects is communications management and proper and timely distribution of information among all internal and external project stakeholders. Despite of emphasizing on the importance of project communications in the literature, there are few studies identifying factors influencing project communications. This research aims to address this shortcoming by identifying and determining sequences and relationships factors affecting project communications and their clustering. The informed communication strategy allows managers to structure the information flow in a better and more controlled manner and to avoid the costs caused by lack of effective and timely communication. The present study is conducted to help clarify the views of the organization's managers and project managers on project communications, and to identify factors affecting it and how they effectively communicate to successfully accomplish the projects. First a number of factors influencing project communications are identified on the basis of previous studies and interviews with experts and project managers working in oil, gas and power plant construction megaprojects in Iran. Then, these factors are analyzed by using the combination of fuzzy DEMATEL and Interpretive Structural Modeling (ISM) techniques. The relationships and sequence between the indicators are determined so that it can be effective in project communications planning and project success through providing an insight for senior managers and project managers.We describe the evolution of a stereotype as it emerged in tweets about the Charlie Hebdo terrorist attack in Paris in early 2015. Our focus is on terms associated with the Muslim community and the Islamic world. The data (400k tweets) were collected via Twitter streaming API and consisted of tweets that contained at least one of 16 hashtags associated with the Charlie Hebdo attack (e.g., #JeSuisCharlie, #IAmCharlie, #ParisAttacks), collected between January 14th and February 9th. From these data, we generated pairwise co-occurrence frequencies between key words such as "Islam", "Muslim(s)", "Arab(s)", and "The Prophet" and possible associates such as "terrorism", "terror", "terrorist(s)", "kill(ed)", "free", "freedom" and "love". We use changes in frequency of co-occurring words to define ways in which acute negative and positive stereotypes towards Muslims and Islam arise and evolve in three phases during the period of interest. We identify a positively-valenced backlash in a subset of tweets associated with the "origins of Islam". Results depict the emergence and transformation of implicit online stereotypes related to Islam from naturally occurring social media data and how pro-as well as anti-Islam online small-world networks evolve in response to a terrorist attack. Modern accelerators have the "flattening filter-free" (FFF) technique to deliver RT with a moderate high-dose rate, currently used in limited clinical indications. No scientifically established data are currently available on the possible effects of this high dose rate on the anti-tumor immune response. We therefore propose here to study these effects in a preclinical CT26 murine colorectal tumor model. , CT26 cells were irradiated on a Varian TrueBeam® linac at 3 different dose rates (4; 12 or 24Gy/min) using the FFF mode. Activation of the anti-tumor immune response was evaluated by the analysis of induction of genes of the type I interferon pathway by RT-qPCR, and by the study of the induction of immunogenic death biomarkers. , an efficacy study of RT delivering 16.5Gy at 2 different dose rates was performed in immunocompetent Balb/c mice carrying CT26 syngeneic tumors, as well as an immunomonitoring analysed by flow cytometry and a transcriptomic analysis using RNA sequencing. Statistical analyzes wies of associations between RT and checkpoint inhibitors fit with this technique of RT. However, further explorations using other cellular models seem to be of interest.Exosomes (EXOs) are naturally occurring nanosized lipid bilayers that can be efficiently used as a drug delivery system to carry small pharmaceutical, biological molecules and pass major biological barriers such as the blood-brain barrier. It was hypothesized that EXOs derived from human endometrial stem cells (hEnSCs-EXOs) can be utilized as a drug carrier to enhance tumor-targeting drugs, especially for those have low solubility and limited oral bioactivity. In this study, atorvastatin (Ato) loaded EXOs (AtoEXOs) was prepared and characterized for its physical and biological activities in tumor growth suppression of 3 D glioblastoma model. The AtoEXOs were obtained in different methods to maximize drug encapsulation efficacy. The characterization of AtoEXOs was performed for its size, stability, drug release, and in vitro anti-tumor efficacy evaluated comprising inhibition of proliferation, apoptosis induction of tumor cells. Expression of apoptotic genes by Real time PCR, Annexin V/PI, tunnel assay was studied after 72 h exposing U87 cells where encapsulated in matrigel in different concentrations of AtoEXOs (5, 10 μM). The results showed that the prepared AtoEXOs possessed diameter ranging from 30-150 nm, satisfying stability and sustainable Ato release rate. The AtoEXOs was up taken by U87 and generated significant apoptotic effects while this inhibited tumor growth of U87 cells. Altogether, produced AtoEXOs formulation due to its therapeutic efficacy has the potential to be an adaptable approach to treat glioblastoma brain tumors.Sepsis is defined as a life-threatening multiorgan dysfunction caused by dysregulated inflammatory response to infection. It remains the primary cause of death from infection if not diagnosed and treated promptly. Therefore, a better understanding of the mechanism for resolving inflammation is needed. Monocytes and macrophages play a pivotal role not only in the induction but also in the suppression of inflammation. However, a tissue-resident macrophage subset that regulates a hyperinflammatory state during sepsis has not been explored. Here we show that CD204+ monocytes and/or macrophages rescued mice from endotoxin-induced septic shock. Serum and tissue proinflammatory cytokine levels were significantly upregulated in the absence of these cells. This study provided evidence that CD204+ monocytes and/or macrophages ameliorate septic shock by suppressing proinflammatory cytokine production.The recommendations for Mycobacterium tuberculosis drug susceptibility testing include both phenotypic and genotypic methods. This concurrent use of differing testing platforms has created an emerging challenge of discordant results, creating a diagnostic dilemma for the laboratorians as well as attending clinicians. We undertook a retrospective study to determine the prevalence of discordant results between the MTBDRplus line probe assay and solid culture-based drug susceptibility testing for rifampicin and isoniazid. The analysis was conducted for the period January 2013 and December 2015 at the Inkosi Albert Luthuli Central Hospital. Rifampicin and isoniazid resistance testing data were "paired" on 8273 isolates for culture-based drug susceptibility testing and line probe assay. The latter method showed high sensitivity and specificity of 93% and 95% respectively for isoniazid testing. For rifampicin testing, sensitivity and specificity were 95% and 75%. Overall, discordance was 14.6% for rifampicin and 7.
